MOSS Conjugation Kits

Moss has bulk and custom service options for conjugate conjugation and manufacturing.

MOSS-Link HRP-IgG Kit

The MOSS HRP-IgG conjugation kit utilizes a novel chemistry to generate highly reproducible IgG-HRP conjugates with a simple procedure. The resulting conjugates have been shown to be extremely stable, retaining 100% activity after storage for 60 days at 37º C with concentrations as low as 0.5 μg/mL.

FEATURES

• Liquid-based reagents-No reconstitution, just Mix and Go!
• Completely scaleable – Conjugate anywhere from 0.1 to 1 gram IgG per reaction.
• Supplies sufficient activated HRP to conjugate all IgG at a 4:1 HRP:IgG ratio.
• Highly efficient HRP incorporation – conjugate purification not usually necessary.
• Customize the HRP:IgG ratio to create optimized conjugates for different applications.
• Conjugates have greatly improved stability vs Lightning-Link™ and other common chemistries.

Each kit includes all reagents needed to conjugate the stated amount of IgG at an HRP-IgG ratio of 4:1.

For details and usage of MOSS-Link HRP and Biotin Labeling kits:
Development of an IL-6 Assay on MagPlex®-Avidin Microspheres using Rapid IgG Biotinylation and RPE-IgG labeling kits

MOSS-Link RPE-IgG Kit

The MOSS RPE-IgG conjugation kit utilizes a novel chemistry to generate highly reproducible IgG-RPE conjugates with a simple procedure. The resulting conjugates have been shown to be extremely stable, retaining nearly 100% activity after storage for 2 months at 37ºC.

FEATURES

• Liquid-based reagents -No reconstitution, just Mix and Go!
• Completely scaleable- Conjugate anywhere from 0.1 to 1 gram IgG per reaction.
• Supplies sufficient activated RPE to conjugate all IgG at a 1:1 RPE:IgG ratio.
• Highly efficient RPE incorporation – conjugate purification not usually necessary.
• Customize the RPE:IgG ratio to create optimized conjugates for different applications.]
• Conjugates have greatly improved stability vs Lightning-Link™ and other common chemistries.

Each kit includes all reagents needed to conjugate the stated amount of IgG at an RPE:IgG ratio of 1:1.

MOSS-Link Biotin Labeling Kit

The MOSS Biotin Labeling kit utilizes a novel chemistry to generate highly reproducible biotinylated conjugates with a simple procedure.

FEATURES

• Can be used with up to 10 mg/ml (1%) BSA as a carrier protein
• Reaction complete in as little as 10 minutes
• Room temperature-stable active biotinylation reagent
• Pre-measured active biotinylation reagent coated onto a tube surface
• Completely scalable: conjugate anywhere from 0.1 to 1 gram IgG per reaction
• Highly efficient Biotin incorporation
• Purification not usually required

Each kit includes all reagents needed to biotinylate the stated amount of IgG and includes a highly pure solution of 100 mg/ml BSA for final formulation.

For details and usage of MOSS-Link HRP and Biotin Labeling kits:
Development of an IL-6 Assay on MagPlex®-Avidin Microspheres using Rapid IgG Biotinylation and RPE-IgG labeling kits

MOSS-Link FITC-IgG Labeling Kit

The Moss IgG FITC Labeling Kit utilizes a novel chemistry to generate highly reproducible fluorescein-labeled IgG with a simple procedure.

FEATURES

• Pre-measured active labeling reagent
• Can be used with up to 10 mg/ml (1%) BSA as a carrier protein
• Completely scalable: conjugate anywhere from 0.1 to 1 gram IgG per reaction
• Highly efficient FITC incorporation
• Optimized FITC:IgG ratio by labeling time and temperature
• Purification not usually required

MOSS-Link AP-IgG Conjugation Kit

Alkaline Phosphatase is widely used as an enzymatic label in immunochemistry assays such as ELISA. Preparing stable and reproducible antibody-AP conjugates is one of the biggest challenges of developing immunoassays. The Moss AP-IgG conjugation utilizes a highly robust chemistry to generate highly reproducible IgG-AP conjugates with a simple procedure. The resulting conjugates have been shown to be extremely stable.

FEATURES

• Liquid-based reagents
• Completely scalable: conjugate anywhere from 25 µg to 1 gram IgG per reaction
• Highly efficient AP incorporation – purification not usually necessary
• Customize the AP:IgG ratio to create optimized conjugates for different applications
• Conjugates have greatly improved stability vs alternative methods

Each kit supplies sufficient activated AP to conjugate all IgG at a 2:1 AP:IgG ratio.

For details and usage of MOSS-Link HRP and Biotin Labeling kits:
Development of an IL-6 Assay on MagPlex®-Avidin Microspheres using Rapid IgG Biotinylation and RPE-IgG labeling kits